تأثیر عصاره مریم گلی (Salvia Officinalis) بر سطوح P53 و کراتین کیناز در دویدن سراشیبی: یک مطالعه متقاطع تصادفی، دوسوکور و کنترل‌شده با دارونما

فرجی, حسن; رحیمی, محمد رحمان; تیموری, شلیر

doi:10.34785/J019.2022.392

تأثیر عصاره مریم گلی (Salvia Officinalis) بر سطوح P53 و کراتین کیناز در دویدن سراشیبی: یک مطالعه متقاطع تصادفی، دوسوکور و کنترل‌شده با دارونما

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

¹ استادیار فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه آزاد واحد مریوان، مریوان، کردستان، ایران.

² دانشیار فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه کردستان، سنندج، کردستان، ایران.

³ کارشناسی ارشد فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه کردستان، سنندج، کردستان، ایران.

10.34785/J019.2022.392

چکیده

تأثیر عصاره مریم گلی (Salvia officinalis) بر سطوح p53 و کراتین کیناز (CK) پس از تمرین برونگرا مشخص نیست. هدف: در مطالعه حاضر، تأثیر عصاره مریم گلی بر سطوح سرمی p53 و CK پس از دویدن در سراشیبی بررسی شد. روش‌ها: 14 مرد سالم (سن، 24.4±3.5 سال؛ شاخص توده بدنی، 22.46±1.5 کیلوگرم بر متر مربع) به طور تصادفی در دو شرایط مریم گلی و دارونما قرار گرفتند. آزمودنی‌ها به مدت دو هفته کپسول‌های 500 میلی‌گرمی عصاره مریم گلی (دو کپسول روزانه) یا دارونما مصرف کردند. پس از 14 روز، آزمودنی‌ها با شیب 12٪ شیب سراشیبی با حدود 70٪ حداکثر ضربان قلب تا زمان خستگی ارادی (30 دقیقه) در سراشیبی دویدن کردند. نمونه‌گیری خون قبل و بلافاصله پس از تمرین دویدن برای اندازه‌گیری غلظت سرمی p53 و CK انجام شد. نتایج: نتایج نشان داد که در شرایط مریم گلی، تغییرات سطح p53 در قبل از تمرین نسبت به بعد از دویدن از نظر آماری معنی‌دار نبود (0.14 = p). در شرایط دارونما، سطح p53 در بعد از دویدن نسبت به قبل از دویدن به طور معنی‌داری افزایش یافت (0.001 = p). همچنین، سطح CK فقط در شرایط دارونما در بعد از دویدن نسبت به قبل از دویدن به طور معنی‌داری افزایش یافت (0.007 = p).

نتیجه‌گیری: یافته‌های مطالعه حاضر نشان داد که دویدن در سرازیری به عنوان انقباض برونگرا ممکن است با افزایش سطح p53 و CK منجر به آپوپتوز سلولی و آسیب عضلانی شود، اما مکمل‌دهی کوتاه‌مدت مریم گلی احتمالاً افزایش آپوپتوز و نشانگر آسیب عضلانی در سرم ناشی از ورزش حاد را مهار می‌کند.

تازه های تحقیق

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Salvia Officinalis Extract on P53 and Creatine Kinase Levels in Downhill Running: A Crossover Randomized, Double-Blind, and Placebo-Controlled Study

نویسندگان [English]

Hassan Faraji ¹
Mohammad Rahman Rahimi ²
Sheler Taeimouri ³

¹ Assistant Professor of exercise physiology, Department of Physical Education, Faculty of Humanities, Marivan Azad University, Marivan, Kurdistan, Iran.

² Associate Professor of exercise physiology, Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Kurdistan, Iran.

³ Master of exercise physiology, Department of exercise physiology،, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Kurdistan, Iran

چکیده [English]

The effect of salvia officinalis (Sage) extract on levels of p53 and creatine kinase (CK) after eccentric exercise is unclear.
Objective: In the present study, the effect of salvia officinalis extract on serum levels of p53 and CK after a downhill running was investigated
Methods: 14 healthy men (age, 24.4±3.5 yr; body mass index, 22.46±1.5 kg/m2) were randomly placed in two conditions of sage and placebo. Subjects took 500 mg capsules of sage extract (two daily) or placebo for two weeks. After 14 days, subjects performed downhill running at 12% downhill grade at about 70% of the maximum heart rate until volitional exhaustion (30 minutes). Blood sampling was performed before and immediately after the running workout for the measurement of the serum p53 and CK concentrations.
Results: The results showed that in the sage condition, the changes in p53 levels in the before exercise compared to the after running were not statistically significant (p = 0.14). In the placebo condition, p53 levels in the post- running increased significantly compared to the pre- running (p = 0.001). Also, CK levels in the post-running only in the placebo condition increased significantly compared to the pre- running (p = 0.007).
Conclusion: The findings of the present study showed that downhill running as eccentric contraction may lead to cell apoptosis and muscle damage by increasing p53 and CK levels, but short-term sage supplementation is likely inhibits increased apoptosis and muscle damage marker in serum induced by acute exercise.

کلیدواژه‌ها [English]

Eccentric contractions
Cellular programming death
Muscle damage
age

اصل مقاله

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