The effect of aerobic training along with black pepper extract consumption on memory disorders markers in rats with Alzheimer's disease

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran

2 Department of exercise physiology, Zand Higher Education Institute, shiraz, Iran.

10.22034/ren.2025.144649.1136

Abstract

Aim: The aim of the present study was to investigate the combined effect of eight weeks of aerobic exercise and black pepper extract consumption on memory disorders markers in rats with Alzheimer's disease (AD).

Method: 30 male Sprague-Dawley rats with AD were randomly divided into AD control, sham, black pepper, exercise, and exercise + black pepper groups. AD induced by injection of 10 mg/kg trimethyltin chloride (n=6). Six rats were also placed in the healthy group. Aerobic exercise was performed on a treadmill for eight weeks, three sessions per week, at a speed of 10 to 24 meters per minute. Black pepper was administered daily at a dose of 100 mg/kg via intraperitoneal injection. 48 hours after the last training session, hippocampal GABA-RA and Aβ gene expression were measured and the results were analyzed with one-way and two-way analysis of variance tests.

Results: In the patient control group, GABA-RA gene expression was significantly lower, and Aβ gene expression was significantly higher than in the healthy control group (P=0.001). GABA-RA gene expression was significantly higher in the exercise + black pepper group than the black pepper, exercise and patient control groups. Aβ gene expression was significantly lower in the exercise + black pepper group than the black pepper, exercise and patient control groups. (P=0.001).

Conclusion: Generally, it seems that aerobic exercise along with black pepper consumption have a synergistic effect in improving memory disorders markers in the AD model. Generalizing these results to the human population requires further studies.

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History

  • Receive Date: 04 October 2025
  • Revise Date: 17 November 2025
  • Accept Date: 25 November 2025
  • First Publish Date: 25 November 2025

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