The Effect of Creatine Supplementation on Some Oxidative Stress Indices and Glutathione Ratio (GSH/GSSG) After Repeated Wingate Tests in Wrestlers

Articles in Press

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Physical Education and Sport Sciences, Faculty of Physical Education, Shahrood University of Technology, Shahrood, Iran.

2 Department of Exercise Physiology, Faculty of Health and Sport Sciences, Shahid Beheshti University, Tehran, Iran.

3 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran

10.22034/ren.2025.144365.1120

Abstract

Aim: The purpose of this study was to investigate the effect of creatine supplementation on malondialdehyde (MDA), glutathione disulfide (GSSG), glutathione (GSH) and the ratio of these two after repeated intensive exercise in wrestlers.

Method: This study utilized a double-blind, placebo-controlled, crossover design. For this purpose, 10 subjects with mean age 22.5±3 years voluntary participated in this study. with subjects participating in both 2 stages of intensive exercise with 15 min Inactive rest. Each stage included three lower and upper extremity Wingate test and with alternative pattern with 1 min active rest between each test. First time creatine or placebo (0.1 gr per kg of body weight) were consumed. Also, the second dose of supplement or placebo was given after the end of second stage of test and third dose was given after the completion of fourth stage. Blood sample was taken before and after and one hour after recovery.

Results: The results of the research using the method of repeated analysis of variance (2x3) showed that the consumption of creatine supplement compared to the placebo prevented the increase in the glutathione disulfide (p=0.008) and malondialdehyde (p=0.001), but the response of the GSH and GSH/GSSG ratio were not related to creatine consumption (p<0.05).

Conclusion: It seems that the creatine supplementation can have a significant effect on some indicators of oxidative stress in wrestlers, but due to the no change in other indicators, further research is suggested.

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Main Subjects

Research in Exercise Nutrition

Articles in Press, Accepted Manuscript
Available Online from 15 October 2025

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History

  • Receive Date: 22 August 2025
  • Revise Date: 08 October 2025
  • Accept Date: 15 October 2025
  • First Publish Date: 15 October 2025

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