The effect of high-intensity interval training and ginger supplementation on poly ADP-ribose polymerase 1 (PARP-1) and sirtuin 1 (SIRT1) gene expression in male rats with nonalcoholic fatty liver disease
Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license
Aim: The aim of the present study was to investigate the effect of high-intensity interval training and ginger supplementation on poly ADP-ribose polymerase 1 (PARP-1) and sirtuin 1 (SIRT1) gene expression in male mice with nonalcoholic fatty liver disease.
Method: Twenty-five adult male Wistar rats, six weeks old and weighing 160±25 grams, were randomly selected and placed on a high-fat diet for 12 weeks to induce nonalcoholic fatty liver disease. The rats were then divided into five groups: healthy control, fatty liver control, exercise, ginger supplementation, and exercise + ginger supplementation. After confirmation of nonalcoholic fatty liver disease, ginger supplementation was administered daily and intense interval training was administered to the relevant groups for 8 weeks, 5 sessions per week. Real Time-PCR was used to measure PARP-1 and SIRT1 gene expression.
Results: The expression of the PARP-1 gene in the exercise, ginger supplement, and exercise and ginger supplement groups was significantly reduced compared to the fatty liver control group (P=0.001). The expression of this gene in the fatty liver control group was also significantly increased compared to the healthy control group (P=0.001). The expression of the SIRT1 gene was also significantly increased compared to the fatty liver control group in the exercise, ginger supplement, and exercise and ginger supplement groups (P=0.001). The expression of this gene in the fatty liver control group was also significantly reduced compared to the healthy control group (P=0.001). No significant difference was observed between the experimental groups (P>0.05).
Conclusion: High-intensity interval training combined with ginger supplementation resulted in decreased PARP-1 and increased SIRT1 in male rats with nonalcoholic fatty liver disease. These changes appear to be effective in improving the severity of the disease.
Cheragh Birjandi, S. , Fakhraei, Y. , Yaghoubi, A. , Rezaeian, N. and Rezaei, V. (2025). The effect of high-intensity interval training and ginger supplementation on poly ADP-ribose polymerase 1 (PARP-1) and sirtuin 1 (SIRT1) gene expression in male rats with nonalcoholic fatty liver disease. Research in Exercise Nutrition, 4(1), -. doi: 10.22034/ren.2025.143968.1112
MLA
Cheragh Birjandi, S. , , Fakhraei, Y. , , Yaghoubi, A. , , Rezaeian, N. , and Rezaei, V. . "The effect of high-intensity interval training and ginger supplementation on poly ADP-ribose polymerase 1 (PARP-1) and sirtuin 1 (SIRT1) gene expression in male rats with nonalcoholic fatty liver disease", Research in Exercise Nutrition, 4, 1, 2025, -. doi: 10.22034/ren.2025.143968.1112
HARVARD
Cheragh Birjandi, S., Fakhraei, Y., Yaghoubi, A., Rezaeian, N., Rezaei, V. (2025). 'The effect of high-intensity interval training and ginger supplementation on poly ADP-ribose polymerase 1 (PARP-1) and sirtuin 1 (SIRT1) gene expression in male rats with nonalcoholic fatty liver disease', Research in Exercise Nutrition, 4(1), pp. -. doi: 10.22034/ren.2025.143968.1112
CHICAGO
S. Cheragh Birjandi , Y. Fakhraei , A. Yaghoubi , N. Rezaeian and V. Rezaei, "The effect of high-intensity interval training and ginger supplementation on poly ADP-ribose polymerase 1 (PARP-1) and sirtuin 1 (SIRT1) gene expression in male rats with nonalcoholic fatty liver disease," Research in Exercise Nutrition, 4 1 (2025): -, doi: 10.22034/ren.2025.143968.1112
VANCOUVER
Cheragh Birjandi, S., Fakhraei, Y., Yaghoubi, A., Rezaeian, N., Rezaei, V. The effect of high-intensity interval training and ginger supplementation on poly ADP-ribose polymerase 1 (PARP-1) and sirtuin 1 (SIRT1) gene expression in male rats with nonalcoholic fatty liver disease. Research in Exercise Nutrition, 2025; 4(1): -. doi: 10.22034/ren.2025.143968.1112
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