نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I
نویسندگان
گروه علوم ورزشی، موسسه آموزش عالی زند شیراز، شیراز، ایران.
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Aim: The study of changes in DNA methylation markers in prostate tissue offers a new research area for the development of epigenetic therapeutic strategies in prostate cancer. The aim of the present study was to investigate the synergistic effect of aerobic exercise and curcumin supplementation on changes in some DNA methylation markers in prostate tissue of rats with prostate cancer.
Method: The statistical sample of the present study was 42 Wistar rats, of which 35 were divided into groups after ensuring the induction of prostate cancer, (1) control-patient, (2) curcumin supplement-patient, (3) aerobic exercise-patient, and (4) curcumin supplement + aerobic exercise-patient, and (5) sham. Also, to investigate the effects of cancer induction on the research variables, 7 rats were considered as a healthy control group. The exercise group performed aerobic exercise for eight weeks. Curcumin supplement was administered to rats at a dose of 160 μL/kg body weight. 8-OHdG levels were measured by 8-OHdG ELISA and O6-methylguanine DNA methyltransferase (MGMT) gene expression was measured by Real-time PCR.
Results: O-6-methylguanine DNA methyltransferase (MGMT) gene expression was significantly increased in the exercise + curcumin supplement -patient group compared to control-patient (P=0.045) and curcumin supplement -patient (P=0.047). No significant difference was observed among the study groups in terms of 8-hydroxy-2-deoxyguanosine (8-OHdG) levels (P=0.25).
Conclusion: The combined supplement + exercise intervention has a greater effect on reducing oxidative stress in prostate cancer than either exercise training or curcumin supplementation.
کلیدواژهها [English]
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